Determination of Total Mercury in Muscle Tissues of Marine Fish Species by Ultrasonic Assisted Extraction Followed by Cold Vapor Atomic Absorption Spectrometry

A simple and rapid ultrasonic assisted extraction procedure (UEP) was developed for the determination of total mercury (Hg) in muscle tissues of marine fish species. For this purpose four fish species were collected from fish markets of Karachi, Pakistan. Total Hg concentration was determined by cold vapor atomic absorption spectrometry (CV-AAS), following UEP. Certified reference material DORM-2 (dogfish muscle) was used to validate the results. No significant difference was observed between the experimental results and the certified values of CRM (paired t-test). The limit of detection (LOD) and limit of quantitation (LOQ) of Hg were 0.133 and 0.445 µg/kg respectively. The Hg concentration in muscle tissues were obtained in the range of 0.721 – 1.41 mg/kg on dry weight. The contribution of the daily intake of Hg, based on the consumption of 250 g fresh fish muscles per day was found in the range of 0.615 – 1.22 µg/kg body weight/ day, which is greater than WHO permissible limit.

  • Abdul Q. Shah National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan
  • Tasneem G. Kazi National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan
  • Jameel A. baig National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Hassan I. Afridi National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Ghulam A. Kandhro National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Sumera Khan National Center of Excellence in Analytical Chemistry, University of Sindh 76080, Jamshoro 76080, Pakistan
  • Nida F. Kolachi National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Sham K. Wadhwa National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan

Abstract

A simple and rapid ultrasonic assisted extraction procedure (UEP) was developed for the determination of total mercury (Hg) in muscle tissues of marine fish species. For this purpose four fish species were collected from fish markets of Karachi, Pakistan. Total Hg concentration was determined by cold vapor atomic absorption spectrometry (CV-AAS), following UEP. Certified reference material DORM-2 (dogfish muscle) was used to validate the results. No significant difference was observed between the experimental results and the certified values of CRM (paired t-test). The limit of detection (LOD) and limit of quantitation (LOQ) of Hg were 0.133 and 0.445 µg/kg respectively. The Hg concentration in muscle tissues were obtained in the range of 0.721 – 1.41 mg/kg on dry weight. The contribution of the daily intake of Hg, based on the consumption of 250 g fresh fish muscles per day was found in the range of 0.615 – 1.22 µg/kg body weight/ day, which is greater than WHO permissible limit.

Published
Dec 1, 2010
How to Cite
SHAH, Abdul Q. et al. Determination of Total Mercury in Muscle Tissues of Marine Fish Species by Ultrasonic Assisted Extraction Followed by Cold Vapor Atomic Absorption Spectrometry. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 11, n. 2, p. 6, dec. 2010. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/102>. Date accessed: 23 nov. 2017.