Separation of Non-alkaloid Toxin Lignans and New Flavonoid from Himalayan Mayapple (Podophyllum Hexandrum Royle) by High-Speed Counter-Current Chromatography and Their Anti-inflammatory Activity Evaluation

Abstract

Podophyllum hexandrum Royle (Berberidaceae) is reported from the Himalayan region and China.
It is also known as the Himalayan Mayapple and is reported for the treatment of constipation,
fever, jaundice, liver disorders, etc. Herein, the isolation of chemical constituents using high-speed
counter-current chromatography (HSCCC) from the EtOH extract of the rhizomes of Himalayan
Mayapple is reported. As a result, kaempferol 3-glucoside (1), quercetin-3-O-β-D-glucopyranoside
(2), quercetin 3-O-β-D-glucopyranosyl-(1→6)-3-O-ethyl-β-D-glucopyranoside (3), kaempferol 3-
O-β-D-glucopyranoside (4), α-peltatin(5), podophyllotoxin (6), 4'-demethylpodophyllotoxin (7),
4',5'-didemethylpodophyllotoxin (8), and kaempferol (9)were separated. Compounds 6-9 were
separated by the normal HSCCC while 1-5 were obtained by the offline-recycling HSCCC using
HEMWat (1:9:4:6, v/v) solvent system. The pure components were tested in lipopolysaccharidesinduced
mice macrophage cells. Compounds 6 and 7 showed significant inhibition. The nitric
oxide production was inhibited by compounds 6 and 7, effectively, with IC50 values of 1.328 x 10-6
and 2.851 x 10-6 M, respectively. In this assay, kaempferol (9), a positive inhibitor expressively
inhibited lipopolysaccharides-induced nitric oxide production.