Separation of Non-alkaloid Toxin Lignans and New Flavonoid from Himalayan Mayapple (Podophyllum Hexandrum Royle) by High-Speed Counter-Current Chromatography and Their Anti-inflammatory Activity Evaluation

  • Iftikhar Ali Karakoram International University, 15100 Gilgit
  • Shahid Aziz
  • Marzia Zahra Department of Chemistry, Karakoram International University, Gilgit 15100, Pakistan
  • Yan Mu
  • Erum Dilshad
  • Meher Ali
  • Xiao Wang

Abstract

Podophyllum hexandrum Royle (Berberidaceae) is reported from the Himalayan region and China.
It is also known as the Himalayan Mayapple and is reported for the treatment of constipation,
fever, jaundice, liver disorders, etc. Herein, the isolation of chemical constituents using high-speed
counter-current chromatography (HSCCC) from the EtOH extract of the rhizomes of Himalayan
Mayapple is reported. As a result, kaempferol 3-glucoside (1), quercetin-3-O-β-D-glucopyranoside
(2), quercetin 3-O-β-D-glucopyranosyl-(1→6)-3-O-ethyl-β-D-glucopyranoside (3), kaempferol 3-
O-β-D-glucopyranoside (4), α-peltatin(5), podophyllotoxin (6), 4'-demethylpodophyllotoxin (7),
4',5'-didemethylpodophyllotoxin (8), and kaempferol (9)were separated. Compounds 6-9 were
separated by the normal HSCCC while 1-5 were obtained by the offline-recycling HSCCC using
HEMWat (1:9:4:6, v/v) solvent system. The pure components were tested in lipopolysaccharidesinduced
mice macrophage cells. Compounds 6 and 7 showed significant inhibition. The nitric
oxide production was inhibited by compounds 6 and 7, effectively, with IC50 values of 1.328 x 10-6
and 2.851 x 10-6 M, respectively. In this assay, kaempferol (9), a positive inhibitor expressively
inhibited lipopolysaccharides-induced nitric oxide production.

Published
Jun 20, 2024
How to Cite
ALI, Iftikhar et al. Separation of Non-alkaloid Toxin Lignans and New Flavonoid from Himalayan Mayapple (Podophyllum Hexandrum Royle) by High-Speed Counter-Current Chromatography and Their Anti-inflammatory Activity Evaluation. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 25, n. 1, p. 81-89, june 2024. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/1095>. Date accessed: 15 july 2024. doi: http://dx.doi.org/10.21743/PJAEC/2024.06.08.