Spectrophotometric Determination of Isoniazid in Dosage forms by Derivatization
Isoniazid (INH) anti-tuberculosis drug was determined spectrophotometrically after derivatizing with 2-hydroxy-1,4-naphthoquinone (HNQ) at pH 3 in aqueous-methanolic solution. The reaction conditions were optimized and the derivative absorbed maximally at 365 nm .The Beer’s law was obeyed in the range 5-25µg/ml. With coefficient of determination 0.9991.The method was applied for the determination of INH in pharmaceutical preparations with coefficient of variation 0.860.98.
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