Spectrophotometric Determination of Isoniazid in Dosage forms by Derivatization

  • F. M. A Rind Faculty of Pharmacy, University of Sindh, Pakistan
  • M. Y Khuhawar Dr.M.A.Kazi Institute of Chemistry, University of Sindh, Pakistan
  • K. F. Almani Faculty of Pharmacy, University of Sindh, Pakistan
  • A.D. Rajpar Department of Chemistry, Shah Abdul Latif University Khairpur, Pakistan

Abstract

Isoniazid (INH) anti-tuberculosis drug was determined spectrophotometrically after derivatizing with 2-hydroxy-1,4-naphthoquinone (HNQ) at pH 3 in aqueous-methanolic solution. The reaction conditions were optimized and the derivative absorbed maximally at 365 nm .The Beer’s law was obeyed in the range 5-25µg/ml. With coefficient of determination 0.9991.The method was applied for the determination of INH in pharmaceutical preparations with coefficient of variation 0.860.98.

Published
Dec 1, 2005
How to Cite
RIND, F. M. A et al. Spectrophotometric Determination of Isoniazid in Dosage forms by Derivatization. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 6, n. 2, p. 5, dec. 2005. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/126>. Date accessed: 22 sep. 2017.