Phenolic Acids Composition of Fruit Extracts of Ber (Ziziphus mauritiana L., var. Golo Lemai)

  • Ayaz Ali Memon National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan
  • Najma Memon National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan
  • Muhammad Iqbal Bhanger National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan
  • Devanand L. Luthria Food Composition and Methods Development Lab, 10300 Baltimore Ave., Human Nutrition Research Center, Bldg. 161 BARC-East Beltsville, MD 20705-2350, USA

Abstract

Fruits of Ziziphus mauritiana L. (ber) are consumed in fresh and dried/processed form in many countries across Asia including Pakistan. In the present study, we analyzed the composition of total phenolic acids (free, soluble-bound and insoluble-bound) from ber fruit extracts by applying a pressurized liquid base hydrolysis extraction (PLBHE) using Dionium cells. Nine phenolic acids (protocatechuic, p-hydroxybenzoic, ferulic, chlorogenic, vanillic, caffeic, vanillin, o- and pcoumaric acids) were extracted, separated, and quantified by HPLC-DAD. Identification of phenolic acids was achieved by comparison of retention times, ultraviolet, and mass spectral data with authentic commercial standards. Results showed that p-coumaric acid (3719 ± 22 µg/g) was the predominant phenolic acid extracted from ber samples. In addition, four phenolic acids, namely p-hydroxybenzoic (2187 ± 71 µg/g), vanillin (2128 ± 20 µg/g), ferulic (2629 ± 96 µg/g), and o-coumaric acids (2569 ± 41 µg/g) were obtained in intermediate amounts from dried Ziziphus mauritiana L. fruit. The total phenolic acids content was determined as 18231 ± 306 µg/g dry matter basis (DMB). This study indicates that ber fruit is a good natural source of phenolic acids and that PLBHE can be used for the assay of phenolic acids.

Published
Dec 3, 2012
How to Cite
MEMON, Ayaz Ali et al. Phenolic Acids Composition of Fruit Extracts of Ber (Ziziphus mauritiana L., var. Golo Lemai). Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 13, n. 2, p. 6, dec. 2012. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/207>. Date accessed: 21 nov. 2017.