HPLC Determination of α-keto Acids from Human Serum Using 2,3-Diamino-2,3-dimethylbutane as Derivatizing Reagent

  • Khalida Parveen Mahar Department of Chemistry, Faculty of Science, Shah Abdul Latif University Khairpur, Pakistan
  • Muhammad Yar Khuhawar Institute of Advanced Research Studies in Chemical Science, University of Sindh, Jamshoro, Pakistan
  • Kulsoom Ubedullah Abbasi Institute of Advanced Research Studies in Chemical Science, University of Sindh, Jamshoro, Pakistan
  • Gulam Qadir Shar Department of Chemistry, Faculty of Science, Shah Abdul Latif University Khairpur, Pakistan
  • Rafia Azmat Department of Chemistry, University of Karachi, Pakistan
  • Jameel Ahmed Baig National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan

Abstract

Seven α-keto acids, pyruvic acid (PYR), 2-oxobutyric acid (KB), 3-methyl-2-oxobutyric acid (MKBA), 3-methyl-2-oxovaleric acid (K3MVA), 4-methyl-2-oxovaleric acid (K4MVA), 2oxoglutaric acid (KG) and Phenyl pyruvic acid (PPY) as derivatives of 2,3-diamino-2,3dimethybutane (DDB) were separated by HPLC column Zorbax C–18 (4.6x150 mm-id). The compounds were eluted with methanol-water-acetonitrile (40:58:2 V/V/V) with flow rate 1 ml/min. UV detection was carried out by photodiode array at 255 nm. Linear calibration plots were obtained with 0.1 to 60 µg/ml with limits of detection (LoD) within 0.04-0.4 µg/ml. The method was applied for the analysis of α-keto acids from serum of diabetic patients with blood glucose level 430-458 mg/dl and healthy volunteers. The amounts of α-keto acids observed 3.249.71 µg/ml with RSD 1.1-1.9% in diabetic patients were higher than healthy volunteer’s 0.11-1.3 µg/ml with RSD 0.9-2.6%. 

Published
Jun 3, 2013
How to Cite
MAHAR, Khalida Parveen et al. HPLC Determination of α-keto Acids from Human Serum Using 2,3-Diamino-2,3-dimethylbutane as Derivatizing Reagent. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 14, n. 2, p. 8, june 2013. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/226>. Date accessed: 04 july 2020.
Citation Formats
Issue
Vol 14 No 2 (2013): Pakistan Journal of Analytical & Environmental Chemistry
Section
Research Articles

Submission of a manuscript to the Journal will be taken to imply that:

  1. The work is original and has not been previously published (except in the form of an abstract or a part of a published lecture, review or thesis).
  2. It is not being considered for publication elsewhere, and
  3. If accepted for publication, it will NOT be published commercially elsewhere in the same form, in any language, without the consent of Editor or Publisher.