Exploration of Mechanistic Insights of Acemetacin in Melanogenesis Through Zebrafish Model, Enzyme Kinetics, Molecular Docking and Simulation Approaches

  • Hussain Raza College of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju-si, Chungnam 32588, Republic of Korea. http://orcid.org/0000-0002-3078-6660
  • Mehar Ali Kazi Institute of Biochemistry, University of Sindh, Jamshoro, 76080, Pakistan.
  • Mubashir Hassan Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore 54000 Pakistan.
  • Qamar Abbas Department of Physiology, University of Sindh, Jamshoro, 76080, Pakistan.
  • Sung-Yum Seo College of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju-si, Chungnam 32588, Republic of Korea

Abstract

The present study describes the anti- melanogenesis effect of Acemetacin (ACE). Essential
protein (melanin) that is vital for the skin for defense from UV rays. In the present research,
emerging drug ACE was examined for its melanin inhibition using three different (in vitro, in vivo
and computational) methods. ACE showed remarkable potency (IC50 = 0.353 ± 0.003 μM) against
tyrosinase in the comparison of standard, kojic acid (IC50 = 16.841 ± 1.161 μM) and ACE
exhibited competitive inhibition. In the in vivo study zebrafish embryos were exposed with 5, 10,
15 and 20 μM of ACE and same doses for positive control (Kojic Acid). At 72 h treatment, ACE
expressively (P<0.001) reduced the level of pigmentation (62.89%) at a concentration of 20 μM,
relative to that of kojic acid (39.64%). The binding profile of ACE was confirmed by molecular
docking and the stability of the docked complexes was justified by MD simulation. Based on our
results, it was concluded

Published
Jun 19, 2020
How to Cite
RAZA, Hussain et al. Exploration of Mechanistic Insights of Acemetacin in Melanogenesis Through Zebrafish Model, Enzyme Kinetics, Molecular Docking and Simulation Approaches. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 21, n. 1, p. 115-124, june 2020. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/585>. Date accessed: 13 july 2020. doi: http://dx.doi.org/10.21743/pjaec/2020.06.14.