Development of Colorimetric Method for the Quantitative Analysis of Amlodipine Besylate in Dosage form Using 4- Dimethyleaminobenzaldehyde as Derivatizing Reagent
The study is based on the determination of amlodipine besylate (AB) after derivatization with 4-dimethylaminobenzaldehyde (DMAB) using UV/Visible spectrophotometer at pH 5 to yield derivative that is measured at λ max 399 nm. The calibration graph obtained was liner and fulfilled Beer Lambert’s law in the concentration range 05-25 μg/mL of (AB) and DMAB having coefficient of determination R² 0.9988 with RSD 0.93% and molar absorptivity 4.04x103 mole-1 cm-1. Quantitative / analytical parameters such as pH, heating time, temperature were optimized. Reagent concentration /volume, interday and intraday studies were also carried out. There was no effect of various solvents and additives observed on the determination of AB in commercially available drugs. The method is stable, accurate, rapid and simple for the study of imine derivative
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