Environmental Friendly Production of Amylase from Aspergillus niger EFRL-FC-024 Using Corn Waste as Carbon Source

Amylase production from Aspergillus niger

  • Abdul-Nabi Mirjatt Institute of Microbiology, University of Sindh, Jamshoro, Pakistan
  • Imrana Khushk Institute of Biotechnology & Genetic Engineering
  • Hiba Zahid
  • Abdul Sattar Qureshi
  • Muhammad Aqeel Bhutto
  • Bushra Watoor Institute of Biotechnology & Genetic Engineering

Abstract

Amylase is an indispensable and industrially important enzyme that hydrolyzes carbohydrates particularly
starch into simple sugars. Amylase enzymes have been isolated from various sources such as microbes,
animals and plants. However, microorganisms are highly preferred as compared to plants and animal
sources. Amylases of fungal origin are highly stable compared to amylases produced by bacterial species.
The aim of this study was to investigate the production of extracellular amylase enzyme from Aspergillus
niger EFRL-FC-024 using sugarcane bagasse and corn waste as an energy source under submerge
fermentation conditions. Primarily, the fungal strain was grown for 6 days using sugarcane bagasse and
corn waste, respectively. Mainly, the growth of a microorganism was also evaluated using different pH,
temperature and incubation periods. The results revealed maximum amylase production of 1.64 U/mL
when A. niger was cultured for 96 h using corn waste. Moreover, addition of different nitrogen sources
showed the highest amylase production when peptone was supplemented as a nitrogen source. Finally, the
effect of pH indicated maximal concentration of amylase enzyme at pH 6.0. The present study will highly
be beneficial to explore the role of fungal strain A. niger in amylase production at Industrial levels.

Published
Jun 23, 2021
How to Cite
MIRJATT, Abdul-Nabi et al. Environmental Friendly Production of Amylase from Aspergillus niger EFRL-FC-024 Using Corn Waste as Carbon Source. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 22, n. 1, p. 165-171, june 2021. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/665>. Date accessed: 29 july 2021. doi: http://dx.doi.org/10.21743/pjaec/2021.06.17.