Analysis of Protein by Spectrophotometric and Computer Colour Based Intensity Method from Stem of Pea (Pisum sativum) at Different Stages

  • Mohammad Saleh Memon Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
  • Afsheen Mushtaque Shah Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
  • Allah Nawaz Memon Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
  • Abdul Wahab Ansari Department of Computer Science, ISRA, University, Hyderabad, Sindh, Pakistan
  • Basir Ahmed Arain Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan

Abstract

In this study proteins were analyzed from pea plants at three different growth stages of stem by spectrophotometric i.e Lowry and Bradford quantitative methods and computer colour intensity based method. Though Spectrophotometric methods are regarded as classical methods, we report an alternate computer based method which gave comparable results. Computer software was developed the for protein analysis which is easier, time and money saving method as compared to the classical methods.

Published
Dec 1, 2010
How to Cite
MEMON, Mohammad Saleh et al. Analysis of Protein by Spectrophotometric and Computer Colour Based Intensity Method from Stem of Pea (Pisum sativum) at Different Stages. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 11, n. 2, p. 10, dec. 2010. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/98>. Date accessed: 15 dec. 2017.