Simultaneous Determination of Orotic Acid and Folic Acid in Pure forms and in Pharmaceuticals Formulations by HPLC
Abstract
Studies were carried out to develop a simple, rapid and accurate HPLC method for the simultaneous determination of Orotic Acid and Folic Acid in pharmaceutical formulation. The separation was done on ODS column by the application of isocratic reversed-phase liquid chromatographic technique. Mobile phase consisted of 800ml phosphate buffer pH 7.2 and 70ml methanol. The method was successfully used for the determination of these drugs in the presence of additives and excipients, which were normally encountered in pharmaceutical formulations. The proposed method of analysis was applied for the individual analysis of both these constituents in their pure forms and found equallyeffective.
Submission of a manuscript to the Journal will be taken to imply that:
- The work is original and has not been previously published (except in the form of an abstract or a part of a published lecture, review or thesis).
- It is not being considered for publication elsewhere, and
- If accepted for publication, it will NOT be published commercially elsewhere in the same form, in any language, without the consent of Editor or Publisher.
