Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R Dye
Determination of Antidepressant Drugs By Oxidation Using Ponceau 4R Dye
Abstract
A simple and sensitive indirect spectrophotometric method is developed for the assay of
Adrenaline, Dopamine hydrochloride, and Methyldopa in their pure and pharmaceutical
preparations. The method is based on the principle of oxidation of the above drugs with a known
amount of the oxidizing agent, N-bromosuccinimide (NBS), in hydrochloric acid. The unreacted
oxidant bleaches Ponceau 4R dye, then the absorbance of the remaining dye (ΔA) is measured at
507 nm. The method obeyed Beer’s law within the concentration range of 3.5-10, 4-15, and
5-20 μg/mL, with molar absorptivity values of 8.01×104, 5.32×104, and 4.93×104 L. mol-1cm-1 for
Adrenaline, Dopamine hydrochloride and Methyldopa, respectively. The recovery ranged between
99.15 to 100.61%, and the relative standard deviation (RSD) was less than 1.42 % for all drug
compounds. The method was applied successfully to pharmaceutical preparations for the studied
medicinal compounds from different origins, as its results were in good agreement with the
original content of the drugs in their pharmaceutical formulations and with the standard addition
procedure, which proved that there was no interference by the excipients.
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