Examination of Impact of Arsenic Stress on Soil Microbes

  • Maleeha Umber Assistant Professor Botany Department, Nusrat Jahan College Rabwah
  • Mirza Naseer Ahmad, Ahmad Professor, Department of Geography, Faculty of Earth Sciences, Abdus Salaam research forum, Nusrat Jahan College Rabwah,Chenab Nagar, Pakistan.
  • Rashida Sultana, Sultana Lecturer, Department of Botany, Faculty of Life Sciences, Abdus Salaam research forum, Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.
  • Fareeha Iffat, Iffat Student BS (Hons.) Botany, Department of Botany,Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.
  • Ayesha Khalid, Khalid Student BS (Hons.) Botany, Department of Botany,Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.
  • Shanza Kiran, kiran Student BS (Hons.) Botany, Department of Botany,Nusrat Jahan College Rabwah, Chenab Nagar, Pakistan.

Abstract

The research was performed at Botany department, Nusrat Jahan College Rabwah Pakistan to screen stress tolerance level of soil microbes (pseudomonas and penicillium) taken from NARC (National Agricultural Research Centre) Pakistan against different levels of sodium arsenite stress (1 mg/L to 10 mg/L). The research was based on completely randomized design. Three drops were applied to microbes grown on CLED (cysteine-, lactose-, and electrolyte-deficient) media to determine zone of inhibition through disk, well and spread method, while control group was without sodium arsenite application. After application of various levels of arsenic stress
organisms were incubated for 24 h at 37°C. After incubation, zones of inhibition were measured. Our study has shown that both micobes cannot overcome higher levels of arsenic stress because in higher stress petriplates, increased inhibitory zones were observed.

Published
Dec 24, 2020
How to Cite
UMBER, Maleeha et al. Examination of Impact of Arsenic Stress on Soil Microbes. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 21, n. 2, p. 342 – 348, dec. 2020. ISSN 2221-5255. Available at: <https://pjaec.pk/index.php/pjaec/article/view/592>. Date accessed: 07 may 2021. doi: http://dx.doi.org/10.21743/pjaec/2020.12.36.