Highly Selective Purification of Grewia asiatica Anthocyanin Based on Macroporous Resins
In the present study, enactments and separation characteristics of five extensively utilized
adsorbents for enhancement and purification of Grewia asiatica Anthocyanins (ACNs) extracts
were analysed. Among five tested resins (AB-8, Sepabeads Sp-700, C18SPE Cartridge, Sepabeads
Sp-207 and AB-80), AB-8 resin exhibited the best adsorbent ability for Grewia asiatica ACNs
(84.24mg/mL resin). Results of static adsorption tests revealed that AB-8 resin selected for
kinetics and isotherm experiments followed a pseudo 2nd order model along with Langmuir
isotherm. In order to improve operational procedure, dynamic adsorption and desorption tests were
done on a packed column of AB-8 resin. Optimum factors for subsequent adsorption-desorption
experiments; processing volume 20ml, flow rate 2 mL/min with elution solvent of acidified
methanol (1%v/v) were used. HPLC and LC-MS/MS profiles of the purified extract confirmed
seven ACNs in Grewia asiatica samples, out of which cyanidin-3-O-(6"acetylglucoside)
comprises 44-63% (695 μg/g) of total ACNs composition. Moreover, pigment purification using
AB-8 resin did not alter ACNs mixture composition after purification but enhance the peak
intensity and gives effective purification. Hence present work reveals that the separation procedure
established through column chromatography providing an effective methodology to enhance the
purification of ACNs from Grewia asiatica.
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