Determination of Lisinopril in Pure and Tablet form by Using 2-Hydroxynaphthaldehyde as Derivatizing Reagent
Lisinopril; Spectrophotometry; 2HNA, derivatization
Abstract
An easy, sensitive and accurate spectrophotometric method has been developed for the
determination of Lisinopril (LNP) in pure and tablet formulations based on derivatization reaction
with 2-hydroxynaphthaldehyde (2HNA). The derivatization reaction was carried out in methanol
solvent at pH-5.5 at 95±2C for 15 min. The linear calibration curve was obtained that obeyed the
Beer’s law within the concentration range 5-50 μgmL-1 of LNP at 433 nm with a coefficient of
determination R²=0.996. The recovery was in the range from 98.25-101.82 with molar absorptivity
of drug 9×103 mole-1cm-1. The method was accurate and precise (intra-day variation 0.05-0.97%
and inter-day 0.07-1.6%), with limit of detection (LOD) and limit of quantification (LOQ) 0.264
μgmL-1 and 0.8 μgmL-1, respectively. No interferences from the excipients were detected. The
method was applied for the rapid analysis of LNP in pharmaceutical products.
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