Development and Validation of HPLC-UV Method for Determination of Paraquat in Raw and Commercial Samples
A new, rapid, efficient, low cost, robust, accurate and reproducible analytical method for the
determination of Paraquat has been developed using reverse phase high performance liquid
chromatography with UV-Visible detector (HPLC-UV). Paraquat is a frequently used herbicide.
This method is useful for the detection and quantification of paraquat in raw materials and
commercial samples with excellent recoveries upto almost 100%. pH of the mobile phase
(acetonitrile:methanol:water 1:1:2) was optimized as 2.5 with ortho-phosphoric acid. This
analytical method was validated with excellent linearity R2 = 0.999. LOD and LOQ were
calculated to be 0.74 mg/L and 2.45 mg/L, respectively. The method showed a high precision
(RSD%) value of 0.32, while the accuracy measured in terms of percentage recovery was almost
100% under optimized conditions. The robustness of the method was studied by changing the flow
rate and the mobile phase concentration ratios, and the results obtained were within the permissible
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