Spectrophotometric Determination of Primaquine by Coupling with Diazotized p-Nitroaniline Application to Pharmaceutical Formulations

  • Ferial Mahmood El-Samman Department of Chemistry, College of Science, Mosul University, Mosul, Iraq
  • Asma Natiq Al-Irhayim Department of Chemistry, College of Science, Mosul University, Mosul, Iraq
  • Enam Ahmed Hamdoon Department of Chemistry, College of Science, Mosul University, Mosul, Iraq

Abstract

A simple spectrophotometric method for the determination of primaquine phosphate has been developed. The method is based on the coupling reaction of primaquine phosphate in acidic medium with diazotized p-nitroaniline, to form a yellow-orange water-soluble azo dye that shows maximum absorption at 482 nm. Beers law is obeyed over the concentration range 5-300 g of primaquine phosphate in a final volum of25ml, i.e., 0.2-12 ppm with a molar absorptivity of 6.6104 l mol-1 cm-1 and Sandells sensitivity index of 0.0068 g.cm-2, a relative error of -0.63 to +0.32% and a relative standard deviation of –0.18 to –2.8%, depending on the concentration level. Interferences due to excipients have been examined. The proposed method has been applied to the assay of primaquine phosphate in pharmaceutical formulations (tablets).

Published
Dec 1, 2011
How to Cite
EL-SAMMAN, Ferial Mahmood; AL-IRHAYIM, Asma Natiq; HAMDOON, Enam Ahmed. Spectrophotometric Determination of Primaquine by Coupling with Diazotized p-Nitroaniline Application to Pharmaceutical Formulations. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 12, n. 1 & 2, p. 6, dec. 2011. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/168>. Date accessed: 21 nov. 2017.