Production of Ethanol by Indigenous Wild and Mutant Strain of Thermotolerant Kluyveromyces Marxianus Under Optimized Fermentation Conditions

  • Shaheen Aziz Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro 76080, Pakistan.
  • Hafeez ur Rehman Memon Institute of Petroleum and Natural Gas Engineering, Mehran University of Engineering & Technology, Jamshoro, 76080, Pakistan
  • Farman Ali Shah Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro 76080, Pakistan.
  • M.I. Rajoka National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Faisalabad, Pakistan.
  • Suhail A. Soomro Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro 76080, Pakistan

Abstract

The maximum ethanol production and β-fructofuranocidase formation  under fermentation studies were carried out in microprocessor controlled  23-L stainless steel fermenter at the following conditions: Temp = 40 ˚C (wild &mutant organism) , pH= 5.5, carbon source (molasses=15% sugar)  nitrogen source  (ammonium sulphate 0.75%), 300 RPM stirring speed and oxygen flow rate was 0.1 vvm. Mutant. Strain of thermotolerant Kluyveromyces marxianus M15 produced maximum production of ethanol at 48hr.All Kinetic Parameters have been studied for the utilization of substrate and production of maximum ethanol for both wild and mutant strains. It has been observed that wild strain was growing up to 550C while the mutant strain was growing up to 650C. In this comparison study, wild and mutant strain showed that Mutant-derived M15 was  stronger over its parental culture due to its more thermal stability and production of ethanol at     65 oC at which wild organism could not grow

Published
Dec 1, 2009
How to Cite
AZIZ, Shaheen et al. Production of Ethanol by Indigenous Wild and Mutant Strain of Thermotolerant Kluyveromyces Marxianus Under Optimized Fermentation Conditions. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 10, n. 1 & 2, p. 9, dec. 2009. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/34>. Date accessed: 21 nov. 2017.