Capillary Gas Chromatographic Determination of Gamma Aminobutyric acid and Putrescine in Cerebrospinal Fluid using Trifluoroacetylacetone as Derivatizing Reagent

  • Liaquat Ali Zardari Department of Chemistry, Shaheed Benazir Bhutto University, Shaheed Benazirabad, Sindh
  • Muhammad Afzal Kamboh Department of Chemistry, Shaheed Benazir Bhutto University, Shaheed Benazirabad, Sindh, Pakistan
  • Hamid Rashidi Nodeh Department of Food Science & Technology, Faculty of Food Industry and Agriculture, Standard Research Institute (SRI), Karaj P.O. Box: 31745-139, Iran
  • Abdul Jabbar Laghari Dr. M. A. Kazi Institute of Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan
  • Muhammad Yar Khuhawar Dr. M. A. Kazi Institute of Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan
  • Suhail Khokhar Department of Electrical Engineering, Quaid-e-Awam University of Engineering, Science & Technology Nawabshah Sindh, Pakistan

Abstract

 determination of gamma aminobutyric acid (GABA) and putrescine (Put) using trifluoroacetylacetone (FAA) as derivatizing reagent from Cerebrospinal Fluid (CSF) samples prior to their gas chromatographic- flame ionization detector (GC-FID) analysis. GABA, Put, cadaverine (Cad) and tyramine (TY) as imitative of FAA extracted from the column HP-5 (30 m x 0.32 mm i.d) at temperature 110 ºC for 1 min, tracked by heating rate 25 ºC to 260 ºC /min. The detection was carried out by FID with segregate ratio 10:1, v/v with whole run time 10 min. The proposed method showed linear calibration range between 2.5-50 µg/mL with low limit of detection 1.0 - 2.5 µg/mL analogous to 0.1 ng to 0.25 ng for selected Put, Cad, GABA, and TY. The method based on the pre-concentration was used for the determination of GABA and Put from CSF of human being and amounts found were 0.25- 0.56 µg/mL and 0.16 - 0.41 µg/mL with relative standard deviation (RSD) within 0.8 - 1.1 and 1.1 - 1.5 %, respectively. Many of aminoacids tested, separated completelyand did not variate the determinations of GABA and Put.

Published
Jun 27, 2018
How to Cite
ZARDARI, Liaquat Ali et al. Capillary Gas Chromatographic Determination of Gamma Aminobutyric acid and Putrescine in Cerebrospinal Fluid using Trifluoroacetylacetone as Derivatizing Reagent. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 19, n. 1, p. 28-35, june 2018. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/389>. Date accessed: 19 july 2018. doi: http://dx.doi.org/10.21743/pjaec/2018.06.02.