Stabilizers Enhanced the Activity of Mutarotase Chracterized from Bovine Kidney Cortex

  • M. ANJUM ZIA Department of Chemistry (Biochemistry), University of Agriculture, Faisalabad, Pakistan
  • SADIA JAVED Department of Chemistry (Biochemistry), University of Agriculture, Faisalabad, Pakistan.
  • KHALIL UR REHMAN Department of Chemistry (Biochemistry), University of Agriculture, Faisalabad, Pakistan.

Abstract

The crude extract of mutarotase having 5.491 U/mL activity (1.264 U/mg specific activity) was subjected to sephadex G-150 column and obtained 16.518 U/mg specific activity. Optimum pH and temperature were 7.5 and 40 0C having maximum activity of 4.273 U/mL and 4.918 U/mL respectively. It was noted that, 200 mg substrate concentration showed highest activity 5.607 U/mL and that of 5.519 U/mL at 500 L enzyme concentration. The study on effect of stabilizers showed that glycerol (15 %) as best one among sodium benzoate and sodium citrate with the activity of 3.71 U/mL even after 30 days.

Published
Jun 1, 2004
How to Cite
ZIA, M. ANJUM; JAVED, SADIA; REHMAN, KHALIL UR. Stabilizers Enhanced the Activity of Mutarotase Chracterized from Bovine Kidney Cortex. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 5, n. 1, p. 4, june 2004. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/47>. Date accessed: 18 oct. 2017.