Stabilizers Enhanced the Activity of Mutarotase Chracterized from Bovine Kidney Cortex
The crude extract of mutarotase having 5.491 U/mL activity (1.264 U/mg specific activity) was subjected to sephadex G-150 column and obtained 16.518 U/mg specific activity. Optimum pH and temperature were 7.5 and 40 0C having maximum activity of 4.273 U/mL and 4.918 U/mL respectively. It was noted that, 200 mg substrate concentration showed highest activity 5.607 U/mL and that of 5.519 U/mL at 500 L enzyme concentration. The study on effect of stabilizers showed that glycerol (15 %) as best one among sodium benzoate and sodium citrate with the activity of 3.71 U/mL even after 30 days.
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