Development and Validation of HPLC-UV Method for Determination of Paraquat in Raw and Commercial Samples

  • Tariq Mahmood Ansari Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan http://orcid.org/0000-0002-8074-3941
  • Ahmed Yar Institute of Chemical Sciences, Bahauddin Zakariya University, Multan
  • Asad Raza Department of Chemistry, Govt. Civil Lines College, Multan
  • Suryyia Manzoor Institute of Chemical Sciences, Bahauddin Zakariya University, Multan

Abstract

A new, rapid, efficient, low cost, robust, accurate and reproducible analytical method for the
determination of Paraquat has been developed using reverse phase high performance liquid
chromatography with UV-Visible detector (HPLC-UV). Paraquat is a frequently used herbicide.
This method is useful for the detection and quantification of paraquat in raw materials and
commercial samples with excellent recoveries upto almost 100%. pH of the mobile phase
(acetonitrile:methanol:water 1:1:2) was optimized as 2.5 with ortho-phosphoric acid. This
analytical method was validated with excellent linearity R2 = 0.999. LOD and LOQ were
calculated to be 0.74 mg/L and 2.45 mg/L, respectively. The method showed a high precision
(RSD%) value of 0.32, while the accuracy measured in terms of percentage recovery was almost
100% under optimized conditions. The robustness of the method was studied by changing the flow
rate and the mobile phase concentration ratios, and the results obtained were within the permissible
accepted values

Published
Jun 29, 2022
How to Cite
ANSARI, Tariq Mahmood et al. Development and Validation of HPLC-UV Method for Determination of Paraquat in Raw and Commercial Samples. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 23, n. 1, p. 148-159, june 2022. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/897>. Date accessed: 14 aug. 2022. doi: http://dx.doi.org/10.21743/pjaec/2022.01.15.