Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) Shells

Authors

  • Ammara Tooba Akbar Department of Biotechnology, University of Karachi, Karachi-75270, Pakistan Author
  • Mahnaz Ahmad Department of Biotechnology, University of Karachi, Karachi-75270, Pakistan Author
  • Rashida Rahmat Zohra Department of Biotechnology, Jinnah University for Women, Karachi-74600, Pakistan Author
  • Raheela Rahmat Zohra Department of Biotechnology, University of Karachi, Karachi-75270, Pakistan Author
  • Ahmad Khan Department of Materials Science and Engineering, Institute of Space Technology, Islamabad-44000, Pakistan Author
  • Muhammad Atiq Ur Rehman Department of Materials Science and Engineering, Institute of Space Technology, Islamabad-44000, Pakistan Author
  • Mohamed Abbas Electrical Engineering Department, College of Engineering, King Khalid University, Abha 61421, Saudi Arabia Author

DOI:

https://doi.org/10.21743/pjaec/2025.06.04

Keywords:

Chitin, Chitosan, Biopolymer, Shrimp shells waste, Purification, DDA

Abstract

Seafood is widely consumed across the globe and serves as a vital source of essential nutrients. However, the processing of seafood generates significant amounts of waste, including shells and scales, which hold potential for the production of valuable byproducts. This study presents an efficient method for synthesizing high-quality chitosan from Pink shrimp (Metapenaeus dobsoni) shells. The extraction process involves a novel two-step purification of chitin, followed by an energy-saving freeze-pump-out-thaw (FPT) cycle and optimized deacetylation to obtain chitosan. Physicochemical characterization revealed that the chitosan has an average molecular weight (Mv) of 620 kDa and is soluble in 1% acetic acid. Scanning electron microscopy (SEM) confirmed the conformation of the synthesized chitosan. The degree of deacetylation was determined to be 97.2% using Fourier-transform infrared spectroscopy (FTIR), while the crystallinity index (Icr) was calculated at 69% via powdered X-ray diffraction (XRD). The findings demonstrated that the chitosan extracted from this crustacean source possesses unique physical and chemical properties, making it highly suitable for applications in biomedical fields and food packaging. 

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Published

2025-06-25

How to Cite

(1)
Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus Dobsoni) Shells. Pak. J. Anal. Environ. Chem. 2025, 26 (1), 39-48. https://doi.org/10.21743/pjaec/2025.06.04.

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