Simpler and Faster Spectrophotometric Determination of Diclofenac Sodium in Tablets, Serum and Urine Samples

Abdul Rauf Khaskheli; Sirajuddin; Kamran Abro; S. T. H. Sherazi; H. I. Afridi; S. A. Mahesar; Munawar Saeed

doi:10.21743/

Authors

Abdul Rauf Khaskheli National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan Author
Sirajuddin National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan Author
Kamran Abro National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan Author
S. T. H. Sherazi National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan Author
H. I. Afridi National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan Author
S. A. Mahesar National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan Author
Munawar Saeed National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan Author

DOI:

https://doi.org/10.21743/

Keywords:

Diclofenac sodium; UV-Visible spectrophotometer; tablets; serum; urine

Abstract

This work describes a simple, sensitive, rapid and economical analytical procedure for direct spectrophotometric evaluation of diclofenac sodium (DS) using aqueous medium without using a chemical reagent. Parameters like time, temperature, acidic and basic conditions and interference by analgesic drugs were studied for a 5μg ml^-1 solution of DS at 276 nm. Under optimized parameters, a linear working range of 0.1–30 g ml^-1 with regression coefficient of 0.9998 and lower detection limit of 0.01 g ml^-1 was obtained. The method was applied for DS contents in tablets, serum and urine samples.