Chromatographic and Spectroscopic Method for Determination of Glycerol in Sanitizer Used During COVID-19 Pandemic

Hemraj Sharma; Hari Prasad Sapkota; Roshani Bhattarai; Nim Bahadur Dangi

doi:10.21743/pjaec/2022.06.10

Authors

Hemraj Sharma Department of Pharmacy, Shree Medical and Technical College, Bharatpur, Chitwan, Nepal Author
Hari Prasad Sapkota Department of Pharmacy, Shree Medical and Technical College, Bharatpur, Chitwan, Nepal Author
Roshani Bhattarai Department of Nursing, Shree Medical and Technical College, Bharatpur, Chitwan, Nepal Author
Nim Bahadur Dangi Pharmaceutical Sciences Program, School of Health and Allied Sciences, Faculty of Health Sciences, Pokhara University, Kaski Nepal Author

DOI:

https://doi.org/10.21743/pjaec/2022.06.10

Keywords:

COVID-19, Sanitizers, Glycerol, 2, 4 Dinitrophenyl hydrazine, Color complex, UV Visible spectroscopy, RP-HPLC

Abstract

The use of sanitizers in the COVID19 pandemic is very common. While using sanitizers in Nepal, we have found people having problems with skin irritation and rashes; hence it was felt to know the amount of glycerol used as an emollient. A validated UV visible spectrophotometric and Reverse-phase High-performance liquid chromatography (RP-HPLC) method was designed to determine the amount of glycerol in the locally available sanitizers. The glycerol in sanitizers showed variation in amount, ranging from 0.78 to 1.66 g and 0.75 to 1.62 g/100 mL by HPLC and UV, respectively. The sanitizer samples with less purity or failed to meet the specification limit should be withdrawn, and their use must be limited. Hence this method seems to be easy, reliable,
and cost-effective for determining glycerol based on the chemical derivatization technique.