Evaluation of the Iodine Concentration in Serum and Urine of Hypothyroid Males Using an Inexpensive and Rapid Method

  • Ghulam Abbas Kandhro National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Tasneem Gul Kazi National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Siraj uddin National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Naveed kazi Liaquat University of Medical and Health Sciences, Jamshoro 76080, Pakistan
  • Hassan Imran afridi National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Mohammad Balal Arain Dept. of Mathematics & Basic Sciences, NED University of Engineering and Technology, Karachi 75270, Pakistan
  • Jameel Ahmed baig National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Abdul Qadir shah National Centre of Excellence in Analytical Chemistry, Sindh University, Jamshoro 76080, Pakistan
  • Nasreen syed Nuclear Institute of Medicine and Radiotherapy (NIMRA) Jamshoro 76080, Pakistan

Abstract

The aim of present study was to evaluate the iodine/iodide status in biological samples (serum and urine) of 172 male hypothyroid patients (HPs) and their supplemental effects on thyroid hormones. For comparison purpose, non-goitrous subjects (n= 220) of same age group and socioeconomic status were also studied. A simple and rapid iodide-ion selective electrode (ISE) was used to measure the concentration of iodine in microwave assisted acid digested serum and urine samples. Quality control for the methodology was established with certified samples and with those obtained by conventional wet acid digestion method on the same certified reference materials (CRMs) and real samples. A linear calibration curve was obtained for a reasonable concentration range of the potassium iodide solutions. The mean concentration of iodine in the serum and urine samples of the HPs was significantly reduced as compared to control male subjects (p< 0.01). The low levels of free triiodothyronine and thyroxin were found in HPs than age matched healthy control (p< 0.005 and 0.002) respectively while high levels of thyroid stimulating hormone were observed in HPs (p< 0.008). The proposed method was relatively efficient as well as cost effective by using inexpensive equipment. It was observed that iodine in biological samples of HPs can play an important role in determining the severity of the hypothyroidism. 

Published
Dec 1, 2009
How to Cite
KANDHRO, Ghulam Abbas et al. Evaluation of the Iodine Concentration in Serum and Urine of Hypothyroid Males Using an Inexpensive and Rapid Method. Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 10, n. 1 & 2, p. 9, dec. 2009. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/24>. Date accessed: 21 nov. 2017.