Production of Ethanol by Indigenous Wild and MutantStrain of Thermotolerant Kluyveromyces Marxianus Under Optimized Fermentation Conditions

Shaheen Aziz; Hafeez ur Rehman Memon; Farman Ali Shah; M.I. Rajoka; Suhail A. Soomro

doi:10.21743/

Authors

Shaheen Aziz Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro 76080, Pakistan Author
Hafeez ur Rehman Memon Institute of Petroleum and Natural Gas Engineering, Mehran University of Engineering & Technology, Jamshoro, 76080, Pakistan Author
Farman Ali Shah Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro 76080, Pakistan Author
M.I. Rajoka National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Faisalabad, Pakistan Author
Suhail A. Soomro Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro 76080, Pakistan Author

DOI:

https://doi.org/10.21743/

Keywords:

Yeast, Fermentation, Kluyveromyces marxianus, metabolic network, thermal effects, Ethanol, Kinetic parameters

Abstract

The maximum ethanol production and â-fructofuranocidase formation under fermentation studies were carried out in microprocessor controlled 23-L stainless steel fermenter at the following conditions: Temp = 40 ˚C (wild &mutant organism) , pH= 5.5, carbon source (molasses=15% sugar) nitrogen source (ammonium sulphate 0.75%), 300 RPM stirring speed and oxygen flow rate was 0.1 vvm. Mutant. Strain of thermotolerant Kluyveromyces marxianus M15 produced maximum production of ethanol at 48hr.All Kinetic Parameters have been studied for the utilization of substrate and production of maximum ethanol for both wild and mutant strains. It has been observed that wild strain was growing up to 550C while the mutant strain was growing up to 650C. In this comparison study, wild and mutant strain showed that Mutant-derived M15 was stronger over its parental culture due to its more thermal stability and production of ethanol at 65 oC at which wild organism could not grow.