Ethanol Production Kinetics by a Thermo-Tolerant Mutant of Saccharomyces Cerevisiae from Starch Industry Waste (Hydrol)

  • Farman Ali Shah Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro, Sindh, Pakistan.
  • Shaheen Aziz Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro, Sindh, Pakistan.
  • Hafeez ur Rahman Memon Institute of Petroleum and Natural Gas Engineering, Mehran University of Engineering & Technology, Jamshoro, Sindh, Pakistan.
  • M.I. Rajoka National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Faisalabad, Pakistan

Abstract

A thermo-tolerant and deoxyglucose-resistant mutant of Saccharomyces cerevisiae was developed and employed to convert them to fuel ethanol in a 150 litre fermenter. Maximum ethanol production was achieved when fermentation of dextrozyme- treated hydrol was carried out for about 36 hours under optimized fermenting conditions. The maximum specific ethanol production rate (qP), and overall ethanol yield (YP/S) were found to be 2.82 g L-1 h-1 and 0.49 g/g respectively. Determination of activation energy for cell growth (Ea= 20.8 kJ/mol) and death (Ed = 19.1 kJ/mol) and product formation and inactivation (EP=35.8 kJ/mol and Edp = 33.5 kJ/mol) revealed the thermo-stability of the organism for up to 47°C.

Published
Jun 1, 2010
How to Cite
SHAH, Farman Ali et al. Ethanol Production Kinetics by a Thermo-Tolerant Mutant of Saccharomyces Cerevisiae from Starch Industry Waste (Hydrol). Pakistan Journal of Analytical & Environmental Chemistry, [S.l.], v. 11, n. 1, p. 6, june 2010. ISSN 2221-5255. Available at: <http://pjaec.pk/index.php/pjaec/article/view/57>. Date accessed: 23 nov. 2017.