Analysis of Protein by Spectrophotometric and Computer Colour Based Intensity Method from Stem of Pea (Pisum sativum) at Different Stages

Authors

  • Afsheen Mushtaque Shah Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan Author
  • Mohammad Saleh Memon Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan Author
  • Allah Nawaz Memon Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan Author
  • Wahab Ansari Department of Computer Science, ISRA, University, Hyderabad, Sindh, Pakistan Author
  • Basir Ahmed Arain Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan Author

DOI:

https://doi.org/10.21743/

Keywords:

Protein, Spectrophotometer, Computer software, Dye and colour intensity

Abstract

In this study proteins were analyzed from pea plants at three different growth stages of stem by spectrophotometric i.e Lowry and Bradford quantitative methods and computer colour intensity based method. Though Spectrophotometric methods are regarded as classical methods, we report an alternate computer based method which gave comparable results. Computer software was developed the for protein analysis which is easier, time and money saving method as compared to the classical methods.

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Published

2010-12-01

How to Cite

(1)
Analysis of Protein by Spectrophotometric and Computer Colour Based Intensity Method from Stem of Pea (Pisum Sativum) at Different Stages. Pak. J. Anal. Environ. Chem. 2010, 11 (2), 63 – 71. https://doi.org/10.21743/.

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