Development and Validation of HPLC Method for the Simultaneous Determination of Insecticides (Carbofuranand Fipronil) in Its Technical and Granular Formulation

Abstract

A precise, accurate, simple and low cost reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous quality assessment of commonly used insecticide carbofuran and fipronil in its technical and formulated products. The
chromatographic parameters, i.e. mobile phase composition, wavelength and flow rate of developed method was optimized in such a way that the HPLC was carried out at 280 nm by using a mobile phase, which contains 80 parts acetonitrile and 20 parts water by volume (ACN:H₂O, 80:20) flowing at a rate of 1.0 mL/min. A linear relationship (R² > 0.999) was shown by calibration curve plotted in the concentration ranged from 30-800 mg/L. The limit of detection (LODs) were found to be 14.5 mg/L and 20.9 mg/L and limit of quantification (LOQs) were recorded to be 43.9 mg/L and 63.2 mg/L for both carbofuran and fipronil respectively. The developed method’s accuracy was exhibited by recovery percentage (96.0-100.8) % of water samples spiked with 100, 300 and 600 mg/L of carbofuran and fipronil, respectively. The developed RP-HPLC method is therefore simple, cheap, rapid and can be proficiently used for the simultaneous identification and quantification of carbofuran and fipronil in its technical and formulated products.